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Home »Botox® Library » Where to Inject Botox® to Treat Frown Lines
📅 Last reviewed: July 10, 2026

Where to Inject Botox® to Treat Frown Lines

A detailed guide to precise Botox® injection points, dosing, and safety techniques for treating glabellar (frown) lines effectively.

Frown lines (glabellar lines) are formed primarily by the glabellar complex—the corrugator supercilii and procerus muscles—which pull the brows inward and downward. Treating these lines with Botox® is one of the most predictable and satisfying aesthetic procedures when anatomy and placement are respected. This guide outlines exactly where to inject, how much to use, and how to stay safe – useful for both new injectors and consumers evaluating treatment plans.
frown lines

Key Takeaways

  • Use the classic 5-point map: 20 Units of Botox® total—4 U into the procerus (1 site) and 4 U into each corrugator at two sites per side (4 sites), all at least 1 cm above the orbital rim.
  • Depth matters: corrugator medial point is deep (to bone), lateral corrugator point is more superficial; the procerus point is intramuscular at midline.
  • Safety first: stay 1–1.5 cm above the superior orbital rim, keep lateral corrugator points conservative to avoid eyelid ptosis, and map while the patient actively frowns.
  • Start conservative, reassess at 2 weeks: most patients do well with 16–24 Units of Botox® in the glabella; titrate by muscle strength, sex, and line severity.

Why treat the glabella (frown lines)?

  • Aesthetic impact: softening the “11s” refreshes the upper face and reduces a tired or stern appearance.
  • Functional balance: reducing brow depressors can subtly lift the medial brow when properly dosed.
  • Predictability: the glabella is among the most reproducible treatment areas for Botox® with a strong safety record when standard mapping is followed.

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Essential anatomy in 60 seconds

  • Procerus: vertical muscle over the nasal bridge that creates horizontal lines at the root of the nose.
  • Corrugator supercilii: paired muscles arising near the medial supraorbital rim, running superolaterally into the mid-brow skin; responsible for vertical “11” lines.
  • Depressor supercilii: small brow depressor that may contribute; typically covered by corrugator points.
  • Nerves/vessels: supratrochlear and supraorbital neurovascular bundles course superior-medially; bruising is possible—use small volumes and gentle pressure afterward.

On‑label glabellar map for Botox®: The 5‑point plan

Total dose: 20 Units of Botox®, divided as follows:
Procerus (1 point): 4 U midline
  • Location: the center of the glabella, at or just above the bony nasal root, where a horizontal crease appears when frowning.
  • Depth: intramuscular, perpendicular to the skin.

Corrugator—right side (2 points): 4 U each

  • Medial point: 1–1.5 cm above the superior orbital rim, just above the medial canthus region where the muscle belly bulges on frown. Depth: deep to bone/periosteum, then withdraw slightly before injection.
  • Lateral point: along the corrugator as it travels superolaterally toward the mid-brow; keep at least 1 cm above the rim and generally medial to the mid‑pupillary line. Depth: more superficial intramuscular/subdermal because the corrugator thins laterally.

Corrugator—left side (2 points): mirror the right side.

Practical mapping tips

1. Medical and Aesthetic History

Always mark with movement: have the patient frown maximally to identify true muscle bellies. Respect margins: stay ≥1–1.5 cm above the orbital rim to reduce toxin diffusion affecting the levator palpebrae superioris (eyelid ptosis risk).

Angle/depth reminders:

  • Medial corrugator: deep deposit (to bone, then slightly withdraw) because the origin is deep.
  • Lateral corrugator: more superficial because fibers insert into dermis.
  • Procerus: intramuscular at midline
Volume per point: small aliquots (for example, 0.1 mL if reconstituted to 4 U/0.1 mL) reduce spread and bruising.

Dosing by patient profile with Botox®

  • First‑time, average female: 16–20 U total (standard is 20 U across 5 points). Consider starting at 16–18 U if brows are heavy or eyelids borderline.
  • Male/strong musculature: 20–24 U total; keep the same 5-point map but consider up‑dosing evenly.
  • Very fine lines or petite anatomy: 12–16 U total with careful placement; plan a 2‑week touch‑up.
  • Static etched lines: standard dosing plus skin therapies (resurfacing, microneedling, topicals). Do not “chase” etched lines with more toxin alone.

Step‑by‑step technique overview

Equipment: 0.3–0.5 mL insulin syringe; 30–32G needle (6–13 mm).

Reconstitution examples for Botox®:

  • 2.0 mL/100 U → 5 U per 0.1 mL
  • 2.5 mL/100 U → 4 U per 0.1 mL (aligns with the 4 U per site label)
  • Patient positioning: seated or semi‑reclined; good overhead lighting.
  • Asepsis: cleanse with alcohol; allow to dry.
  • Injection order: procerus first, then corrugators (medial deep points, then lateral superficial points).
  • Hemostasis: gentle pressure; avoid massage.
  • Photos: capture at rest and maximal frown for documentation

Safety checklist to avoid complications

  • Eyelid ptosis: most often from too‑low or too‑lateral corrugator injections. Solution: keep ≥1–1.5 cm above the rim, avoid injecting below the brow, and keep lateral points conservative.
  • Asymmetry: map and dose evenly; address pre‑existing asymmetry with small adjustments, not big changes.
  • Headache/tightness: usually transient; small aliquots and even distribution help.
  • Bruising: minimize passes, use fine needles, and apply brief pressure.

How the glabella interacts with the forehead

  • Balanced upper face: treating only the forehead can risk brow heaviness; treating only the glabella can create a subtle medial lift and often improves aesthetics on its own.
  • Common combined plan: Glabella 20 U of Botox® + conservative forehead dosing placed high in the frontalis for line softening while preserving brow position.

Aftercare and follow‑up

  • Onset: 3–5 days; peak at ~14 days.
  • Duration: ~3–4 months; stronger muscles may wear off faster.
  • Immediate care: avoid rubbing, facials, helmets, or strenuous exercise for ~4 hours; no lying flat for 4 hours.
  • Review at 2 weeks: adjust with small add‑ons (for example, 1–2 U of Botox® to the most active points). Do not add more if heaviness is present—allow partial wear‑off and reassess.

Special situations and adjustments

  • Heavy medial brows or mild dermatochalasis: favor procerus and medial corrugator points but keep totals conservative; avoid low placement.
  • Short forehead/low brow position: be extra cautious if also treating the frontalis; the glabellar map remains standard but keep corrugator points high.
  • Prior eyelid surgery or history of ptosis: document carefully, use conservative dosing, and maintain safe margins.

Contraindications and cautions

  • Pregnancy or breastfeeding.
  • Active skin infection at injection sites.
  • Known hypersensitivity to any component of Botox®.
  • Neuromuscular junction disorders (e.g., myasthenia gravis, Lambert‑Eaton).
  • Medications that may potentiate effect (e.g., aminoglycosides): weigh risks and benefits.

Documentation essentials (clinic operations)

  • Consent covering indications, expectations, risks (including ptosis), and off‑label nuances if applicable.
  • Pre‑treatment photos (rest and maximal frown).
  • Injection map: exact sites, side, depth notes, and dose per point.
  • Product details: dilution, total Units of Botox®, lot number, and expiry.
  • Aftercare instructions and scheduled 2‑week follow‑up.

For consumers: what an ideal frown line treatment looks like

  • Your provider maps injections while you actively frown and explains why each point is chosen.
  • Doses are precise (often 20 Units of Botox® across five points) and placed safely away from the eyelids.
  • A 2‑week check is offered to fine‑tune with small touch‑ups instead of “overdoing it” on day one.
  • Photos, documentation, and clear aftercare are standard.

Conclusion

The safest, most effective way to treat frown lines is to use the on‑label 5‑point glabellar map with Botox® and respect depth and distance: deep medially, superficial laterally, and always ≥1–1.5 cm above the orbital rim. Start with 16–24 Units of Botox® tailored to muscle strength, then reassess at 2 weeks. This approach delivers natural, reliable softening while minimizing the risk of eyelid ptosis and asymmetry.
Disclaimer: The information provided here is for general knowledge only and should not be considered medical advice. For any questions or concerns about your health or medications, please consult your physician or healthcare provider. They are best equipped to provide guidance specific to your medical needs.

FAQ

Frequently Asked Questions

How many units of Botox® are used to treat frown lines?

The standard on-label dose is 20 Units total, using the 5-point glabellar map: 4 U into the procerus and 4 U into each of two corrugator points per side. Most patients do well with 16–24 U depending on muscle strength, sex, and line severity.

Where exactly are the 5 injection points located?

One point at the procerus (midline, over the nasal bridge) and two points per side in the corrugator supercilii — a medial point near the medial canthus and a lateral point along the muscle as it runs toward the mid-brow. All points should be at least 1–1.5 cm above the superior orbital rim.

How do I avoid causing eyelid ptosis?

Ptosis is usually caused by injecting too low or too lateral. Stay at least 1–1.5 cm above the orbital rim, avoid injecting below the brow, and keep lateral corrugator points conservative.

How deep should each injection go?

The medial corrugator point is injected deep (to bone, then slightly withdrawn) since the muscle originates deep. The lateral corrugator point is more superficial, as fibers insert closer to the dermis. The procerus point is intramuscular at the midline.

When will results appear, and how long do they last?

Onset is typically 3–5 days, peaking around 14 days. Results generally last 3–4 months, though patients with stronger muscles may see effects wear off sooner.

Should the glabella be treated alone or with the forehead?

Treating the glabella alone often creates a subtle medial brow lift and can improve aesthetics on its own. If combining with the forehead, use conservative dosing placed high in the frontalis to preserve brow position.

Is a follow-up appointment necessary?

Yes — a 2-week review is recommended. Small touch-ups (often 1–2 U) can be added to under-treated points, but if any heaviness is present, it’s best to let it partially wear off before adjusting further.

Who shouldn’t receive Botox® for frown lines?

Contraindications include pregnancy or breastfeeding, active skin infection at the injection site, known hypersensitivity to Botox® components, and neuromuscular junction disorders such as myasthenia gravis or Lambert-Eaton syndrome.

Can Botox® fully remove deeply etched frown lines?

Static, etched-in lines respond best to standard dosing combined with skin therapies like resurfacing or microneedling — adding more toxin alone won’t resolve lines that are already permanently set into the skin.

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